JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ682797.1
Database:	nt	Go to original record
Description:	Goodenia pterigosperma voucher UPS Gustafsson &38 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Goodenia pterigosperma
Original size:	920 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	526	+	525 bp

Amplicon:

525 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaacccggg aattaataaa
101   aaggggcaat cctgagccaa atcgcgtttt cagaaaacaa ccaaagtcag
151   aaagcgcaaa tcaaaaggat aggtgcagag actcgatgga agctgttcta
201   acgaatggag tgaattgcct tgcattggta taggaatcct tatatataaa
251   tttaagaaaa gatgaagtat atctataata tctataatat atgcataata
301   gaatatagaa gaattgttgg gaatcgattc catatcggaa tattcatcga
351   tcaaatcatt cactcccgaa tctgatagat ctttatatga aaatatgaaa
401   ttaataggac gagaataaag atagagtcct cttctacatg tctataccgg
451   caataatgaa aattatagta aaaggaaaat ccgttgattt caaaaaatcg
501   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	525	506	2.36e-08

Match details