JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ682658.1
Database:	nt	Go to original record
Description:	Goodenia convexa voucher CBG 8905662 Smith 777 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Goodenia convexa
Original size:	928 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	522	+	521 bp

Amplicon:

521 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaacccggg aactaataaa
101   aaggggcaat cctgagccaa atcgcgtttt cagaaaacaa ccaaagtcag
151   aaagcgcaaa tcaaaaggat aggtgcagag actcgatgga agctgttcta
201   acgaatggag ttaaatgcct tgcgttggta gaggaatcct tatatcgaaa
251   tttcagaaaa gatgaagtat aacttaatat ctataatata tgcataatag
301   aatatgaata taatatagaa gaattgttgg gaatctattt catatcgaaa
351   tattcatcaa tcaaatcatt cactccataa tctgatagat cttttcttaa
401   tcggacgaga ataaagatag agtcctcttc tacatgtcta taccggcaat
451   aatgaaaatt atcgtaaaag gaaaatccgt tgatttcaaa aaatcgtgag
501   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.34e-08
primerB	100.0	20 bp	0	0	521	502	2.34e-08

Match details