JRC GMO-Amplicons | EURL GMFF

Record ID:	JF904765.1
Database:	nt	Go to original record
Description:	Poa arcuata voucher Peterson 16564 (RSA) trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Poa arcuata
Original size:	1801 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	825	1437	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttcgaaa
251   ttctaaaaag aagggcttta tacagctaat aaacacgtat agatactgag
301   atagcaaacg attaatcaca gagcccataa tattggttct ttattctttt
351   ttagaatgaa atttgaaata gaaatgatta tgaaataaaa aattcataat
401   ttttttagaa ttattgtgaa tccattccaa tcgaatattt agtaatcaaa
451   tccttcagtt caaagttttg aggtctttaa aaaagtggat taatcggacg
501   aggacaaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details