JRC GMO-Amplicons | EURL GMFF

Record ID:	JF904754.1
Database:	nt	Go to original record
Description:	Puccinellia frigida voucher Peterson 15769 (RSA) trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Puccinellia frigida
Original size:	1683 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	817	1395	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacctgg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttcgagaagg aagggcttta tacatctaat aaacacctat acccatatta
301   taatataggt tctttattct tttttagaat caaatagaaa tgattatgaa
351   ataaaaaatt cataattttt ttagaattat tgtaaatcca ttcccatcga
401   atatttagta atcaaatcct tcaattcaaa gttttgaggt ctttaaaaaa
451   gtagattaat cgaacgagga caaagagaga gtcccattct acatgtcaat
501   actgacaaca atgaaatttc tagtaaaagg aaaatccgtc gactttataa
551   gtcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details