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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: AB684631.1
Database: nt Go to original record
Description: Cloning vector pSolycp00006 DNA, complete sequence.
Taxon: Cloning vector pSolycp00006
Original size: 11781 bp

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 4103 4297 + 195 bp
Amplicon: 195 bp 
1     gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatatctc cactgacgta agggatgacg cacaatccca ctatc

View other 3571 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 1.44e-06
primerB 100.0 20 bp 0 0 195 176 9.28e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.