Match details

Record ID: AB684629.1
Database: nt Go to original record
Description: Cloning vector pSolycp00004 DNA, complete sequence.
Taxon: Cloning vector pSolycp00004
Original size: 14312 bp

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Method: QL-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 6757 6878 + 122 bp

Amplicon: 122 bp
1     ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gaccttcctc tatataagga
101   agttcatttc atttggagag ga 

View other 819 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.82e-09
primerB 100.0 25 bp 0 0 122 98 1.28e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.