JRC GMO-Amplicons | EURL GMFF

Record ID:	JF791675.1
Database:	nt	Go to original record
Description:	Bouteloua chondrosioides voucher RSA:Columbus 2422 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Bouteloua chondrosioides
Original size:	1327 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	414	984	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctttttttg aaaaaacaag cggttctcaa
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gaggtgtaat tacgttgtgt tggtagtgaa actccctcta
251   aattactaaa ttagagaaag aagggcttta tacatctaat acacacgtat
301   agatactgat atagcaaacg attaatcaca gaacctatac cataatatag
351   attctttctt tatttttttg aatgaaatca ggaatgatta tgaaatagaa
401   aattagaaaa ttcctatcat tgtatttgat atctattaaa aataggatta
451   atcggacgag gataaagaga gagtcccatt ctacatgtca atgctgacaa
501   caatgaaatt tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details