JRC GMO-Amplicons | EURL GMFF

Record ID:	HQ721913.1
Database:	nt	Go to original record
Description:	Solanum coagulans isolate LB2733 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Solanum coagulans
Original size:	1647 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	634	1196	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggactcttta catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatactata
301   tcaactgatt aatgacgacc cgaatccgta ttttttctat aaaaaataga
351   agaattggtg cgaatccatg aagaaagaat cgaatattca ttgatcaaat
401   cattcactcc atagtctgat agatcttttg aagaactgat taatcggacg
451   agaataaaga tagagtcccg ttctacatgt caataccggc aacaatgaaa
501   tttatagtaa aaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details