JRC GMO-Amplicons | EURL GMFF

Record ID:	JN161146.1
Database:	nt	Go to original record
Description:	Polygonum polygamum voucher FLAS:C. Notis 300, D. Tilley tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Polygonum polygamum
Original size:	1006 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	606	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaattg atttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaac tccttcttta caaaaggaag aaaacggata
151   ggtgcagaga ctcaatggaa gctgttctaa caaatggggt tgacagtttt
201   gcgttgttat aagaattgtt ccatcgaaat tccaaaaagg atgaagaata
251   aacctagccg catacgtact caaaatctaa agaatactct atttataatt
301   atataaaaat attcaaatta aatgaaaaaa tattgatgac gaccaaaatc
351   tttattttat gactatgaaa aagtgaaaga attgttgtaa atcgattcca
401   agtttaagaa agaatcgaat attcgttttt tagtcaatta aaacatttat
451   tccccaatcg gatagatctt ttgaaaaccc gacccatcga atgagaatga
501   agatagagtc ccattctacg tgtcaatcct gacaacaatg aaatttagag
551   taagaggaaa atccgtcgac ttttaaaatc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details