JRC GMO-Amplicons | EURL GMFF

Record ID:	JN161144.1
Database:	nt	Go to original record
Description:	Dentoceras myriophylla voucher FLAS:S.F. Brockington 117, C.E. Edwards tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Polygonum dentoceras
Original size:	1007 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	612	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttaattg atttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaac tccttctttc caaaaggaag aaaacggata
151   ggtgcagaga ctcaatggaa gctgttctaa caaatggagt tgacagtttt
201   gcgttgttat aagaattgtt ccatcgaaat tccaaaaagg atgaagaata
251   aacctagtcg catacgtact aaaaatctaa agaatactct atttagatct
301   agaattatat aaaaatattc aaattaaatg aaaaaatatt gatgacgacc
351   aaaatcttta ttttatgact atgaaaaagt ggaagaattg ttgtaaatcg
401   attccaagtt taagaaagaa tcgaatattc gttttttagt caattaaaac
451   atttattccc caatcggata gatcttttgg aaacccgacc catcgaatga
501   gaatgaagat agagtcccat tctacgtgtc aatcctgaca acaatgaaat
551   ttagagtaag aggaaaatcc gtcgactttt aaaatcgtga gggttcaagt
601   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details