JRC GMO-Amplicons | EURL GMFF

Record ID:	JF951094.1
Database:	nt	Go to original record
Description:	Phalaris aquatica isolate AQU284200 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Phalaris aquatica
Original size:	1657 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	753	1369	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtattttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatt gagttaatta cgttgtgttg ttagtggaac tccttccaaa
251   tttgagaaag aagggcttta tacatctaat aaacacgtat agatactgac
301   atacatagca aacgattaat cacagaaccc atattataat ataggttctt
351   tattcttttt tagaatgaaa ttagaaatga ttctgaaata aaaaattctg
401   aatttttttt agaattattg tgaatccatt ccaatcgaat attgagtaat
451   caaatccttc aattcaaagt tttgagatct ttaaaaaagt ggattaatcg
501   gacgaggaca aagagagagt cccattctac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details