JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ417597.1
Database:	nt	Go to original record
Description:	Asarum tamaensis tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Asarum tamaense
Original size:	1012 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	606	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattcaaaat
101   gggcaatcct gagccaaatc cagttttcag aaaacaaaca aaagttcata
151   aagcgagaat caaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatag ggttgacttt actacatcgg taggtagaga aatccttcta
251   tcgaaactcc ggaaaggagg accctagata cgtacgtata ctactgaaat
301   atcaaatatt aatcacgacc ctaatctata ttctatattt ttctatattt
351   tatttttttt tatatgacaa atttaagaat tcttgtgaat ttatttcaga
401   aggaagaatc gaatattcag ttcttcaaaa gatctatcac accagagtct
451   gatagatctt ttgaagaact gattaattgg acgagaataa agatagagtc
501   ccattctaca tgtcaatact gacaacaatg caatttatag taagaggaaa
551   atccgtcgac tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details