JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ417596.1
Database:	nt	Go to original record
Description:	Asarum nipponicum tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Asarum nipponicum
Original size:	1006 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	608	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tggtaacttc caaattcaga gaaaccctgg aattcaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaacaaaca aaagttcata
151   aagcgagaat caaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatag ggttgacttt actacatcgg taggtagaga aatccttcta
251   tcgaaactcc ggaaaggagg accctagata cgtacgtata ctactgaaat
301   ataaaatatt aatcacgacc ctaatctata ttctatattt ttctatattt
351   tatttttttt tttatatgac aaatttaaga attcttgtga atttatttca
401   gaaggaagaa tcgaatattc agttcttcaa aagatctatc acaccagagt
451   ctgatagatc ttttgaagaa ctgattaatt ggacgagaat aaagatagag
501   tcccattcta catgtcaata ctgacaacaa tgcaatttat agtaagagga
551   aaatccgtcg actttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details