Match details

Record ID: AY328168.1
Database: nt Go to original record
Description: Vatica mangachapoi specimen-voucher Jian 2001112901 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon: Vatica mangachapoi
Original size: 1030 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 580 + 580 bp

Amplicon: 580 bp
1     cgaaatcggt agacgctacg gacttaattg tattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   ggcaatcctg agccaaatcc tgtttaagtt caaaaagaaa taggataggt
151   gcagagactc aatggaagtt gttcgaacaa atggggttga ctgcttttgc
201   gttggtaaaa gaatacttct atcgaaactt cagcaaggat aaacatatag
251   aatacataat gaaaaaacta tttcaaaaaa gacgactggg acacctattc
301   ttttgatttc tattttttta taggttatag caaaaagaat tatatgaaaa
351   atcaaaagaa tccttgtgaa tcgatttcaa attgaagaaa gaatcaaata
401   gaatattcac taatcaaaca attcactcca tagtctgata aatcaataga
451   ttaattggac gagaataaag atagagtccc attctacatg tcaatatcaa
501   taccgacaac aatgaaattt atagtaagag gaaaatccgt cgactttaaa
551   aatcgtgagg gttcaagtcc ctctatcccc  

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.61e-08
primerB 100.0 20 bp 0 0 580 561 2.61e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.