JRC GMO-Amplicons | EURL GMFF

Record ID:	AY328167.1
Database:	nt	Go to original record
Description:	Hopea hainanensis specimen-voucher Jian 2001100402 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Hopea hainanensis
Original size:	1062 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	604	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttaatta tattgagcct tggtatggaa
51    acctactgag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   ggcaatcctg agccaaatcc tgtttaaaaa agcgttcaaa aagaaatagg
151   ataggtgcag agactcaatg gaagttgttc gaacaaatgg ggttgactgc
201   ttttgcgttg gtaaaagaat acttctatcg aaacttcggc aaggataaac
251   atatagaata cgtaatgaaa aactatctca aaaaaaacga ctgggacacc
301   tattcttttg atttatattt ttttataggt tatagcaatt tttataggtt
351   atagcaaaaa gaattatatg aaaaatcaaa agaatcggtg tgaatcgatt
401   tcaaattgac gaaagaatcg aatagaatat tcattaatca aaccattcac
451   tccatagtct gataaatcaa tggattaatt ggacgagaat aaagatagag
501   tcccattcta catgtcaata tcaataccga caacaatgaa atttatagta
551   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
601   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details