Match details

Record ID: AY328166.1
Database: nt Go to original record
Description: Muntingia calabura specimen-voucher Jian 2001071010 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon: Muntingia calabura
Original size: 997 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 556 + 556 bp

Amplicon: 556 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagkatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agaataaacc gagactaaac
151   aaaggttcag aaagcgagaa taaaaaaaaa aggataggtg cagagactca
201   atggaagctg ttctaataaa tggcgttgac ggcctttgcg ttagtaaagg
251   aatccttcta tcgaaactac agagaaagaa agggtgaagg ataaacctat
301   atatacatac gtaatgaaaa acgatctcaa aaaaaaaaag aacgggtgtg
351   aatcgaatta aaaattaatg aatataatat tcattaatta aatgattcac
401   tccatagtct gataaatcgt tttaaaaatt gattaatcgg acgagaataa
451   agatagagtc ccattctaca tgtcaatagc gaccccaatg aaatttatag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.50e-08
primerB 100.0 20 bp 0 0 556 537 2.50e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.