Match details

Record ID: AY328164.1
Database: nt Go to original record
Description: Pterospermum heterophyllum specimen-voucher Jian 2001071005 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon: Pterospermum heterophyllum
Original size: 1070 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 613 + 613 bp

Amplicon: 613 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctattatttt acgaaaataa acaaaggttc
151   agcaagcgag aataataaaa aagggatagg tgcagagact caatggaagc
201   tattctaaca aatggggttg actgttggta aaggaatcct tatatcgaaa
251   ctccagaaag gaaaggatgc aggatatacc tatataaaat aaataggtat
301   actaatgaag aactatttca aaaaagacga cctgaacccg tatttttttt
351   atttatatgc aaaataaatt taatttatat gaaaaataaa aagaatcgat
401   tccaaggtga agaaagaatc aaatagaata ttcattaatc aaatcattca
451   ctccatagtc tgataaatct tttgaaaaac tgattaatcg gacgagaata
501   aagatagagt cccgttctac atgtcaatat caataccgac aacaatgaaa
551   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
601   tccctctatc ccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.76e-08
primerB 100.0 20 bp 0 0 613 594 2.76e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.