JRC GMO-Amplicons | EURL GMFF

Record ID:	AY328163.1
Database:	nt	Go to original record
Description:	Excentrodendron hsienmu specimen-voucher Jian 200052706 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Excentrodendron hsienmu
Original size:	1059 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	610	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg ggcttaattg gattgagcct tggtatggaa
51    accyaytaag tgataacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctattatttt acgaaaataa acaaaggttc
151   agcaagcgag aataataaaa aaaggatagg tgcagagact caatggaagc
201   tattctaaca aatggggttg actgttggta aaggaatcct tatatcgaaa
251   ctccagaaag gatgcaagat atacctatat aaagtaaata ggtatactaa
301   tgaaaaacta tttcaaaaaa gacgacctga acccgtattt ttttttattt
351   atatgaaaaa tcaattttat ttatatgaaa aataaaaaga atcgattcca
401   aggtaaagaa agaatcaaat agaatattca ttaatcaaat cattcactcc
451   atagtctgat aaatctttat gaaaaactga ttaatcggac gagaataaag
501   atagagtccc gttctacatg tcaatatcaa taccgacaac aatgaaattt
551   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details