JRC GMO-Amplicons | EURL GMFF

Record ID:	AY328162.1
Database:	nt	Go to original record
Description:	Burretiodendron esquirolii specimen-voucher Tang 99121403 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Burretiodendron esquirolii
Original size:	1063 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	614	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg ggcttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctattatttt acgaaaataa acaaaggttc
151   agcaagcgag aataataaaa aaaggatagg tgcagagact caatggaagc
201   tattctaaca aatggggttg actgttggta aaggaatcct tatatcgaaa
251   ctccagaaag gatgcaagat atacctatat aaagtaaata ggtatactaa
301   tgaaaaacta tttcaaaaaa gacgacctga acccgtattt tttttttatt
351   atttatatga aaaatcaatt ttatttatat gaaaaataaa aagaatcgat
401   tccaaggtaa agaaagaatc aaatagaata ttcattaatc aaatcattca
451   ctccatagtc tgataaatct ttatgaaaaa ctgattaatc ggacgagaat
501   aaagatagag tcccgttcta catgtcaata tcaataacga caacaatgaa
551   atttatagta agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details