JRC GMO-Amplicons | EURL GMFF

Record ID:	AY328161.1
Database:	nt	Go to original record
Description:	Craigia yunnanensis specimen-voucher Gong 99122212 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Craigia yunnanensis
Original size:	1102 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	615	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctattatttt aggaaaataa acaaaggttc
151   agcaagcgag aataataaaa aaaaggatag gtgcagagac tcaatggaag
201   ctattctaac aaatctaaca aatggggttg actgttggta aaggaatcct
251   tatatcgaaa ctccagaaag gatgcaagat atacctatat aaaataaata
301   ggtatactaa tgaaaaacta tttcaaaaaa gacgaaccga acccgtattt
351   tttttttatt tatatgcaaa atcaatttat atgaaaaata aaaagaatcg
401   attccaagtt gaagaaagaa tcgaatagaa tattcattaa tcaaatcatt
451   cactccatag tctgataaat cttttgaaaa actgattaat cagacgagaa
501   taaagataga gtcccgttct acatgtcaat atcaataccg acaacaatga
551   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details