Match details

Record ID: AY328160.1
Database: nt Go to original record
Description: Hainania trichosperma specimen-voucher Tang 99121404 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon: Diplodiscus trichospermus
Original size: 1061 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 612 + 612 bp

Amplicon: 612 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatagaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctattatttt acgtaaaata aaataaacaa
151   aggttcagca agcgagaata agaaaaaaag gataggtgca gagactcaat
201   ggaagctatt ctaacaaatg gggttgactg ttggtaaagg aatccttata
251   tcgaaactcc agaaaggatg caagatatac ctatataaaa taaataggta
301   tactaatgaa aaactatttc aaaaaagacg acccgaaccc gtattttttt
351   tttttattta tatgcaaaat caatttatat gaaaaataaa aagaatcgat
401   ttcaagttga agaaagaatc gaatagaata ttcattaatc aaatcattca
451   ctccatcgtc tgataaatct tttgaaaaac tgattaatcg gacgagaata
501   aagatagagt cccgttctac atgtcaatat caataccgca acaatgaaat
551   ttatagtaag aggaaaatcc gtcgacttta aaaatcgyga gggttcaagt
601   ccctctatcc cc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.76e-08
primerB 100.0 20 bp 0 0 612 593 2.76e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.