JRC GMO-Amplicons | EURL GMFF

Record ID:	AY328157.1
Database:	nt	Go to original record
Description:	Firmiana major specimen-voucher Gong 200061101 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Firmiana major
Original size:	1073 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	618	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggga
51    acctactaag tgatctcgtg tcaccagttc agagataccc tggaatgaaa
101   aatgggcaat cctgagccaa atcctattat tttacgaaaa taaacaaagg
151   ttcagcaagc gagaataata aaaaaaggat aggtgcagag actcaatgga
201   agctattcta acaaatgggg ttgactgttg gtaaaggaat ccttatatcg
251   aaactccaga aaggatgcaa gatataccta tataaaataa ataggtatac
301   taatgaaaaa ctatttcaaa aaaaacgacc cgaacccgta tttttttttt
351   atttatatgc aaaatcaatt tatatgaaaa ataaaaagaa ttgttgtgaa
401   tcgattctaa gttgaagaaa gaatcgaata gaatattcat taatcaaatc
451   attcactcca tagtctgata aatcttttga aaaactgatt aatcggacga
501   gaataaagat agagtcccgt tctacatgtc aatatcaata ccgacaacaa
551   tgaartttat agtaagagga aaatccgycg actttaaaaa tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details