JRC GMO-Amplicons | EURL GMFF

Record ID:	AY328156.1
Database:	nt	Go to original record
Description:	Firmiana platanifolia specimen-voucher Ye 99120502 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Firmiana simplex
Original size:	1076 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	621	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggga
51    agtcyactaa gcgatcactg tcaccarttc agagaaaccc tggaatgaaa
101   aatgggcaat cctgagccaa atcctattat tttacgaaaa taaacaaagg
151   ttcagcaagc gagaataata aaaaaaggat aggtgcagag actcaatgga
201   agctattcta acaaatgggg ttgactgttg gtaaaggaat ccttatatcg
251   aaactccaga aaggatgcaa gatataccta tataaaataa ataggtatac
301   taatgaaaaa ctatttcaaa aaaaacgacc cgaacccgta tttttttttt
351   tttatttata tgcaaaatca atttatatga aaaataaaaa gaattgttgt
401   gaatcgattc taagttgaag aaagaatcga atagaatatt cattaatcaa
451   atcattcact ccatagtctg ataaatcttt tgaaaaactg attaatcgga
501   cgagaataaa gatagagtcc cgttctacat gtcaatatca ataccgacaa
551   caatgaaatt tatagtaaga ggaaaatccg ycgactttaa aaatcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details