Match details

Record ID: AY328148.1
Database: nt Go to original record
Description: Chorisia speciosa specimen-voucher Jian 2001071003 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon: Ceiba speciosa
Original size: 1077 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 616 + 616 bp

Amplicon: 616 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctattatttt acgaaaataa acaagggttc
151   agcaagcgag aataataaaa aaaaggatag gtgcagagac tcaatggaag
201   ctattctaac aaatggggtt gactgttggt aaaggaatcc ttatatcgaa
251   actccagaaa ggatgcaaga tatacctata taaaataaat aggtatacta
301   atgaaaaact atctcaaaaa agacgacccg aacctggatt ttctttttat
351   ttatatgcaa aatcaattta tatgaaaaat aaaaagaatt gttgtgaatc
401   gattccaagt tgaagaaaga atcgaataga atattcatta atcaaatcat
451   tcaccccata gtctgataaa tcttttgaaa aactgattaa tcggacgaga
501   ataaagatag agtcccgttc tacatgtcaa tatcaatacc gacaacaatg
551   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
601   aagtccctct atcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.78e-08
primerB 100.0 20 bp 0 0 616 597 2.78e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.