Match details

Record ID: AY328146.1
Database: nt Go to original record
Description: Pachira macrocarpa specimen-voucher Jian 2001071002 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon: Pachira macrocarpa
Original size: 1103 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 620 + 620 bp

Amplicon: 620 bp
1     cgaaatcggt agacgctacg gacttaattg gattggattg agccttggta
51    tggaaaccta ctaagtgata actttcaaat tcagagaaac cctggaatga
101   aaaatgggca atcctgagcc aaatcctatt attttacgaa aataaacaaa
151   ggttcagcaa gcgagaataa taaaaaaaag gataggtgca gagactcaat
201   ggaagctatt ctaacaaatg gggttgactg ttggtaaagg aatccttata
251   tcgaaactcc agaaaggatg caagatatac ctatataaaa taaataggta
301   tactaatgaa aaactatctc aaaaaagacg acccgaacct ggattttttt
351   ttatttatat gcaaaatcaa tttatatgaa aaataaaaag aattgttgtg
401   aatcgattcc aagttgaaga aagaatcgaa tagattattc attaatcaaa
451   tcattcactc catagtctga taaatctttt gaaaaactga ttaatcggac
501   gagaataaag atagagtccc gttctacatg tcaatatcaa taccgacaac
551   aatgaaattt atagtaagag gaaaatccgt cgacttttaa aatcgtgagg
601   gttcaagtcc ctctatcccc  

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.79e-08
primerB 100.0 20 bp 0 0 620 601 2.79e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.