JRC GMO-Amplicons | EURL GMFF

Record ID:	AY328142.1
Database:	nt	Go to original record
Description:	Hibiscus rosa-sinensis specimen-voucher Ye 2000031214 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Hibiscus rosa-sinensis
Original size:	1054 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	599	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg aacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctattatttt acgaaaataa acataaacaa
151   aagttcagca agcgagaata ataataataa aggaaaggat aggtgcagag
201   actcaatgga agctattcta acaaatgggg ttgactgttg gtaaaggaat
251   ccttatatcg aaactccgga taggtatact aacgaaaaac tatctcaaaa
301   aagacgaccc gaacccgtat tttttttata tgcaaaatct atttatatga
351   aatatgaaaa ataaaaagaa ttgttgtgaa tcgattccaa gttgaagaaa
401   gaatcgaata gaatattcat taatcaaatc attcactcca tagtctgata
451   aatcttttga aaaaactgat taatcggacg agaataaaga tagagtcccg
501   ttctacatgt caatatcaat accgacaaca atgaaattta tagtaagagg
551   aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details