JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319189.1
Database:	nt	Go to original record
Description:	Anaxagorea javanica tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Anaxagorea javanica
Original size:	970 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	583	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattcaaaat
101   gggcaatcct gagccaaatc ctgtgttcaa aaaacaaggg ttcataaagc
151   aagaataaaa aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg gagttaactg cattggtaga ggaattcttt ccttctatcc
251   aaactacaga aaggatgacc ttgtatacgt acgtatacat actgaaatat
301   caaacgatta atcacgactc gaatccgtat ttttcttttt ttctatgaaa
351   aaatttcaga gtagaatgat tgtgaaccga ttccaagttg aaggaagaat
401   cgaatattca aagatcaaat cattcattcc cgagtctaat agttctttta
451   aagaactgat taatcggacg agaataaaga tagagtcccg ttctacatgt
501   caatacagac aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
551   ataaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details