JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319188.1
Database:	nt	Go to original record
Description:	Anaxagorea luzonensis tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Anaxagorea luzonensis
Original size:	962 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	586	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattcaaaat
101   gggcaatcct gagccaaatc ctgtgttcaa aaaacaaggg ttcataaagc
151   aagaatcaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatggagtt aactgcattg gtagaggaat tctttccttc tatccaaact
251   acagaaagga tgaccttgta tacgtacgta tacatactga aatatcaaac
301   gattaatcac gactcgaatc cgtatttttc tttttttcta tgaaaaaatt
351   tcagaataga atgattgtga accgattcca agttgaagga agaatcgaat
401   attcagagat caaatcattc attcccgagt ctaatagttc ttttaaactt
451   ttaaagaact gattaatcgg acgagaataa agatagagtc ccgttctaca
501   tgtcaataca gacaacaatg aaatttatag taagaggaaa atccgtcgac
551   tttataaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details