JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319163.1
Database:	nt	Go to original record
Description:	Pseuduvaria pamattonis tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Pseuduvaria pamattonis
Original size:	979 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	576	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgagaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgtgttctg aaaacaaggt tcagaaagcg
151   agaatcaaaa acagaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaata gagttgattg cattggtagg ggaattcttt ccttctatcc
251   aaacgacaga aaggatgacc ttgtatacgt acgtatacat actgaaacat
301   caaacgatta atcacgactc gaatcagtct ttttttatat gaaaaattga
351   agaatagaag gattgtgatt tgattccaag ttgaaggaag aatcgaatat
401   tcagagatca aatcattcat tccgagtcta atagatcttt agaataactg
451   attaatcgga cgagaataaa gatagagtcc cattctacat gtcaatacag
501   acaacaatga aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details