JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319140.1
Database:	nt	Go to original record
Description:	Polyalthia littoralis tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Marsypopetalum littorale
Original size:	990 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	577	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgagaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgtgttcag aaaacaaggt tcagaaagtg
151   agaatcaaaa acagaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaata gagttgactg cattggtagg ggaattcttt ccttctatcc
251   aaacgacaga aaggatgacc ttgtatacat acgtatacat actgaaacat
301   caaacgatta atcacgactc gaatcagtct ttttttatat aaaaaatttc
351   agaatagaag gattgtgatt tgattccaag ttgaaggaag aattgaatat
401   tcagagatca aatcattcat tcccgagtct aatagatctt tagaataact
451   gattaatcgg acgagaataa agatagagtc ccattctaca tgtcaataca
501   gacaacaatg aaatttatag taagaggaaa atccgtcgac tttaaaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details