JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319137.1
Database:	nt	Go to original record
Description:	Polyalthia glauca tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Maasia glauca
Original size:	966 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	572	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgagaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgtgttcag aaaacaaggt tcagaagaat
151   caaaaacaga aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatagaatt gactgcattg gtgggggaat tctttccttc tatccaaacg
251   acagaaagga tgaccttgta tacgtacgta tacatactca aacatcaaac
301   gattaatcac gactcgaatc agtatttttt tttatgaaaa atttcagaat
351   agaacgattg tgaattgatt ccaagttgaa ggaagaatcg aatattcaga
401   gatcaaatca ttcattcccg agtctaatag atcttttgaa gaactgatta
451   atcgaacgag aataaagata gagtcccatt ctacatgtca atacagacaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details