JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319135.1
Database:	nt	Go to original record
Description:	Polyalthia discolor tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Maasia discolor
Original size:	976 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	575	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgagaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgtgttcag aaaacaaggt tcagaagaat
151   caaaaacaga aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatagaatt gactgcattg gtgggggaat tctttccttc tatccaaacg
251   acagaaagga tgaccttgta tacgtacgta tacatactga aacatcaaac
301   gattaatcac gactcgaatc agtatttttt tttttatgaa aaatttcata
351   atagaaggat tgtgaattga ttccaagttg aaggaagaat cgaatattca
401   gagatcaaat cattcattcc cgagttctaa tagatctttt gaagaactga
451   ttaatcgaac gagaataaag atagagtccc attctacatg tcaatacaga
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt taaaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details