JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319127.1
Database:	nt	Go to original record
Description:	Platymitra macrocarpa tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Platymitra macrocarpa
Original size:	977 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	578	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgagaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgtgttcag aaaacaaggt tcagaaagcg
151   agaatcaaaa acagaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaata gagttgactg cattggtaag ggaattcttt ccttctatcc
251   aaacgacaga aaggatgacc ttgtatacgt acgtatacat actgaaacat
301   caaacgatta atcacgactc gaatcagtct ttttttatat gaaaaaattt
351   cagaatagaa ggattgtgat ttgattccaa gttgaaggaa gaatcgaata
401   ttcagagatc aaatcattca ttcccgagtc taatagatct ttagaatatc
451   tgattaatcg gacgagaata aagatagagt cccattctac atgtcaatac
501   agacaacaat gaaatttata gtaagaggaa aatccgtcga ctttaaaaat
551   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details