JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319100.1
Database:	nt	Go to original record
Description:	Miliusa longipes tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Miliusa longipes
Original size:	958 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	563	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctactaag tgagaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctgtgttcag aaagcgagaa tcaaaaacag
151   aaaaaggata ggtgcagaga ctcaatggaa gccgttctaa caaatagagt
201   tgactgcatt ggtaggggaa ttctttcctt ctatccaaac gacagaaagg
251   atgaccttgt atacgtacgt atacatactg aaacatcaaa cgattaatca
301   cgactcgaat cagtcttttt ttctatgaaa aatttcagaa tagaaggatt
351   gtgatttgat tccaagttga aggaggaatc gaatattcag agatcaaatc
401   attcattccc gagtctaata gatctttaga ataactaatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aatacagaca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details