JRC GMO-Amplicons | EURL GMFF

Record ID:	AY319095.1
Database:	nt	Go to original record
Description:	Mezzettia parviflora tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Mezzettia parviflora
Original size:	953 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	573	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgagaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctgtgttcag aaaacaaggt tcagaaggcg
151   agaatcaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatagaagtt gactgcattg gtagaggaat tctttccttc tatccaaact
251   acagaaagga tgaccttgta tacgtacgta tacatactga ttaatcaaac
301   gattaatcac gactcgaatc cgtatttttt ttttatgaaa aatttcagaa
351   tagaaggatt gtgaatcgat tccaagttga aggaagagtc gaatattcag
401   agatcaaatc attcattccc gagtctaata gatcttttga agaaccgatt
451   aatcggacga gaataaagat agagtcccgt tctacatgtc aatacagaca
501   acaatgaaat ttatagtaag aggaaaatcc gtcggcttta taaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details