JRC GMO-Amplicons | EURL GMFF

Record ID:	AY507532.1
Database:	nt	Go to original record
Description:	Haplomitrium mnioides tRNA-Leu gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe gene, partial sequence; chloroplast.
Taxon:	Haplomitrium mnioides
Original size:	717 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	576	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttgatcc gattgagctt tgggtaagaa
51    attgactaac tgaccgtttt cagattcggg gaagtctagg ttgaataaag
101   aaatagatga tcctgagcta aatctcgttt ttcgtttcca gaacgagata
151   agtgcagaga ctcgaaggaa actatcctaa cgacgaaaaa tcactcgaag
201   ttccgcacgg ggcttttgca acggatgtag taattgccac taatccctat
251   aaacaaaaaa aaaaagggct cgctgggtag tgagtagggg cacctaccgc
301   ggtggtatga tccggtcgat taggtaatta cacccggcag ggagtggttt
351   cgtctccacc cccctccccc ccccaaaaaa aaagaagaat aaaattttct
401   ttgagtatat tttggttcga cagtcccttc cgggcggtgg aggatggaga
451   tggagtcggt cgaggacgaa gatagagtcc gttcttacac gtgatttccg
501   gcggcagtgc aaattgcagt agaaagaaaa tccgttggct ttttaagacc
551   atgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details