JRC GMO-Amplicons | EURL GMFF

Record ID:	AY437943.1
Database:	nt	Go to original record
Description:	Luzula campestris tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Luzula campestris
Original size:	966 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	628	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttaatta ttgagcctta gtatggaaac
51    ctgctaagtg ataacttcca aattcagaga aaccctggaa tccaaaatgg
101   gcaatcctga gccaaatctt aatctggaga aaacttgttt ctctataaaa
151   actagaatca aaaaggataa aggataggtg cagagactca acggaagctg
201   ttctaacgaa tcgaatgact acgttgcatt ggtagctccc tatcgaaata
251   agagacaggg tggtcctata taccaaaagc atatgtatat atatactgaa
301   ttaatcaaat gattaatcac aatcaattct attcttcatg tttataatca
351   aattatgaat gattataaaa tagcaaattc ggaaaaatta tcaattatgg
401   tgaatcagtt ccaagtgaag ctgaatgaaa gaagaattct atattcagta
451   atcaaataat tcatttccag gtttgttagg tcttttgaaa aagtgatgaa
501   tcagacgaga ataaagagag agtccattct acgtgtcaat attgacaaca
551   atgaaattta tagtaagagg aaaatccgtc gattttctag atcgtgaggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details