Match details
| Record ID: |
AY342213.1 |
| Database: |
nt |
Go to original record |
| Description: |
Glycine max lectin (Le2) mRNA, partial cds. |
| Taxon: |
Glycine max |
| Original size: |
741 bp |
View other 2 matches for this record
| Method: |
QT-TAX-GM-004 |
Go to GMOMETHODS record |
| Description: | Quantitative PCR method for detection of soybean lectin gene (Hird et al., 2003). |
| Target: | soybean lectin |
View other 19 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
18 |
87 |
+ |
70 bp |
|
1 tggtcgcgcc ctctactcca cccctatcca catttgggac agcgaaatcg
51 gcagcgttgc cagcttcgcc
View other 3 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
18 bp |
0 |
0 |
1 |
18 |
4.19e-08 |
| primerB |
100.0 |
16 bp |
0 |
0 |
70 |
55 |
4.65e-07 |
| probe |
88.5 |
26 bp |
3 |
0 |
51 |
26 |
2.54e-07 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
