Match details

Record ID: AY342212.1
Database: nt Go to original record
Description: Glycine max lectin (Le2) gene, complete cds.
Taxon: Glycine max
Original size: 1010 bp

View other 3 matches for this record


Method: QT-TAX-GM-001 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of soybean lectin gene
Target:soybean lectin

View other 39 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 2 289 369 + 81 bp

Amplicon: 81 bp
1     tccaccccta tccacatttg ggacagcgaa accggcagcg ttgccagctt
51    cgccgcttcc ttcaacttca ctgtttatgc c 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerB 87.5 24 bp 3 0 81 58 3.05e-06
probe 95.7 23 bp 1 0 30 52 4.80e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.