JRC GMO-Amplicons | EURL GMFF

Record ID:	AY756935.1
Database:	nt	Go to original record
Description:	Banara axilliflora tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Banara axilliflora
Original size:	618 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	617	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttaatcg aattgagcct tagtatggaa
51    acttactaag tgacaacttt caaattcaga gaaaccccgg aaaaaaaggg
101   gcaatcctga gccaaatcct atttttcgaa aacaaacaaa ggttcataaa
151   gacagaatcg gaatacaaaa ggataggtgc agagactcaa tggaagttat
201   tctaacaaat agagttgact gcgttgcatt agtcaagtaa aggaatcctt
251   ctgttaaagt gaaaattcca gaaaaagaga aaaagaaagt taaagtaaag
301   cataacccta tatacataat acataggtat acgtactgaa atactatctc
351   aaatgattaa tgacagccga cccgaatcta tattttaatt ttattatatt
401   tatatgaaaa attgctttga tttgaatcga ttccaagttg acgagaggat
451   cgaatattaa ttgatcagat cattcacccc gtagtctgct gattaatcga
501   acgagagtaa agatagagtc ccattctaca tgtcaatatc gacaacaaag
551   aaatttatag taaaaggaaa atccgtcgac tttataaatc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details