JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ131556.1
Database:	nt	Go to original record
Description:	Anacardium occidentale tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Anacardium occidentale
Original size:	530 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	530	+	530 bp

Amplicon:

530 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt cgaattcaga gaaaccctgg aatcaaaaat
101   gggcaatcct gagccaaatc ctattttacg agaacaaaaa caaacagggg
151   gtcagaacgg gagaaaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgat tgcctttttg ggggaaagaa gggaattctt
251   ctatcgaata tcgaaaggtt ataaaggatg aaggataagc ctatattaca
301   ctatgtataa gtaatgaaaa aatacactat gtatacgtaa tgaaaaagga
351   tctcaaaaat gatgacccga atcctttttg tttttctttt gaagaactaa
401   ttaatcggac gagaataaag atagagtccc attctacatg ccaatatcaa
451   tactggcaac aatgaaattt atagtaagag gaaaatccgt cgactttata
501   aatcgtgggg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	530	511	2.38e-08

Match details