JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ131547.1
Database:	nt	Go to original record
Description:	Glycine max tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Glycine max
Original size:	584 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	584	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagtct tggtatggaa
51    acttaccaag tgagaacttt caaattcaga gaaaccctgg aattcacaaa
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga aaagttcata
151   aagtgataat aaaaaaggga taggtgcaga gactcaatgg aagctgttct
201   aacaaaagga gttgacgatt tttccttttt gcattaggaa aataatagaa
251   tccttccgtc aaaattccag gaatggatca aagataaaca tatatatact
301   gaaatagtat ttcaattgat taatgaagat ccatttgtga taaaaatatt
351   cacaaatgaa agatgtgaat caaatcaatt ccaagttgaa gaaaagatgg
401   aatattcatt gatcaaatta ttcactccat cataatctga tagatccctt
451   gaagaactga tccatcagac gagaataaag atagagtcct attctacatg
501   tcaataccga caacaatgaa atttatagta agaggaaaat ccgtcgactt
551   aagaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details