JRC GMO-Amplicons | EURL GMFF

Record ID:	MT980792.1
Database:	nt	Go to original record
Description:	Lycopus lucidus var. hirtus chloroplast, complete genome.
Taxon:	Lycopus lucidus var. hirtus
Original size:	152096 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46659	47216	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgccggt agaggaatct ttccgtggaa actttcgaaa
251   ggatgaagga taaacgcatc tattgaatac tatatcaaac gattaatgtt
301   ggcccgaatc tgttttttta atatgaaaat aaaaaaaata ggtgtgaatt
351   tatttcacgt tgaagaaaaa atcgaatatt catcaactca ttcactccat
401   agtccgatag atcttttaaa gaacggatta atcggacgag aataaagata
451   gagtcccatt ctacatgcta catgtcaata ccggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details