JRC GMO-Amplicons | EURL GMFF

Record ID:	MK954109.1
Database:	nt	Go to original record
Description:	Lilium pumilum chloroplast, complete genome.
Taxon:	Lilium pumilum
Original size:	152591 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45083	45696	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttttaa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagttgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   attcttctat caaaattaca gagaggatgc ccgccctata tacgtatata
301   tacatactga ccatagtaaa cgattgatta atcgcggccc gaatccatta
351   taatatatat atatatatat gaaaaattta agagttattg taaatctatt
401   ccatattcca atcaaagttt aaggaagaat cgaatattca gtgatcaaat
451   tattcattcc agagtctgta tagatctttt taaaaactga ttattcggac
501   gagaataaag agagagtccc attctacgtg tcaataccga caacaatgaa
551   atttatagta aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details