JRC GMO-Amplicons | EURL GMFF

Record ID:	MT950760.1
Database:	nt	Go to original record
Description:	Alloteropsis cimicina chloroplast, complete genome.
Taxon:	Alloteropsis cimicina
Original size:	139335 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48442	49054	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaaaaaat aagtggttgt
151   caaactagaa cccaaaggaa aaggataggt gcagagactc aatggaagct
201   gttctaacga atcgaagtaa ttacgttgtg ttggtagtgg aactccctcg
251   aaattagaga aagaagggct ttatacatct aatacacacg tatagatact
301   gacatagcaa acgattaatc acagaaccca tatcataata taggttcttt
351   attttttaga atgaaattag gaatgattat gaaatagaaa attctgaatt
401   tttttagaat tattgtgaat ccatttcaat cgaatattga gtaatcaaat
451   ccttcaattc atagttttcg agatctttaa aaaagtggat taatcaaacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details