JRC GMO-Amplicons | EURL GMFF

Record ID:	MT634145.1
Database:	nt	Go to original record
Description:	Salvia rosmarinus plastid, complete genome.
Taxon:	Salvia rosmarinus
Original size:	151695 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46521	47087	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgccg gtagaggaat ctttccatgg aaactttaga
251   aaggatgaag gataaacgca tctatttaat actatatcaa atgattaatg
301   ttgacccgaa tctgtttttt taatatgaaa atgagaaaat ggaaaaatag
351   gtgtgaattt atttcacgtt gaagaataaa tcgaatattc atcaactcat
401   tcactccata gtccgataga tcttttaaag aacttattaa tcggacgaga
451   ataaagatag agtcccgttc tacatgctac atgtcaatac cggcagcaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details