JRC GMO-Amplicons | EURL GMFF

Record ID:	MT937187.1
Database:	nt	Go to original record
Description:	Mazus pumilus voucher PDBK 2008-1240 chloroplast, complete genome.
Taxon:	Mazus pumilus
Original size:	152847 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46685	47251	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   agtgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaagaaaa ggataggtgc agagactcaa tggaagctat
201   tctaacaaat ggagttgact gcattggtag aggaatcttt ccatcaaaac
251   tgcagaaagg atgaaggata aacgtatcta ttgaatacta tatcaattaa
301   tgatggccca aatctgtatc tgtatttttt aatctgaaaa gtggaagaat
351   tggtgtgaat tgattccaca ttgaagaaag aatcgaatat taattcatca
401   aatcattcac tccatagtcc gacagatctt ttaaagaact tattaaatcg
451   ggcgagaata aagatagagt cccattctac atgtcaatat tggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga cttttaaaat cgtgagggtt
551   caagtccctc tatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details