JRC GMO-Amplicons | EURL GMFF

Record ID:	MT948145.1
Database:	nt	Go to original record
Description:	Phryma leptostachya subsp. asiatica voucher PDBK 2008-0813 chloroplast, complete genome.
Taxon:	Phryma leptostachya subsp. asiatica
Original size:	153324 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47969	48529	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aacgggcaat cctgagccaa atcctctttt ctcaaaagaa aggttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gagtaatctt tccatcaaaa cttcagaaag
251   gataaaggat aaacgtatct atcgaatact atatcaaatg attaatgata
301   gcccgaatct gtatctgtat tttttaatat gaaaaatgga agaattggtg
351   tgaattgatt ccacattgaa gaaagaatcg aatattcatt catcaaatca
401   ttcactccat agtccgatag gtcttttaaa gaaatgatta atcggacgag
451   aataaagata gagtcccgtt ctacatgtca ataccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttta aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details