JRC GMO-Amplicons | EURL GMFF

Record ID:	MK433200.1
Database:	nt	Go to original record
Description:	Chelidonium majus chloroplast, complete genome.
Taxon:	Chelidonium majus
Original size:	159741 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49263	49859	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaacaaaaa aagtttctga
151   aagcgagaat aaaataaaaa agggataggt gcagagactc aatggaagct
201   gttctaatga atggagttga ctgcgttgcg ttggtagagg aatctttcta
251   ttgaaacttc agaaagaatg aaggatgaac ctatatacga tacatacgta
301   tacgtactga aatatcaaac gattaatgac gccccaaatc tgtatttttt
351   tatatgaaaa atggaagaat tcttgtgaat caattccaag ttgaaggaag
401   agaagaatcg aatattcagt gatcaaataa ttcgctccat agtctgatag
451   atcttttgac gaactgatta atcagacgag aataaagata gagtcccgtt
501   ctacatgtca atatcaatac cgacaacaat gaaatttata gtaagaggaa
551   aatccgtcga ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details