JRC GMO-Amplicons | EURL GMFF

Record ID:	BK010739.1
Database:	nt	Go to original record
Description:	TPA: Nicotiana obtusifolia chloroplast, complete genome.
Taxon:	Nicotiana obtusifolia
Original size:	156022 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49281	49851	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctttttt ccgaaaacaa acaaaagttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaata cgttggtaaa ggaatcttta catcgaaatt tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatactata
301   tcaaatgatt aatgatgacc cgaatccata ttttttctat aaaaaataga
351   agaattggtg tgaatcgatt ctacattgaa gaaagaatcg aatattcatt
401   gatcaaatca ttcactccat agtctgatag atcttttgaa gaactgatta
451   atcggacgag aataaagata gagtcccgtt ctacatgtca ataccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details